| Polymerase chain reaction (PCR) is construed as | | | | laboratory technique based on the polymerase |
| one of the pioneering molecular biology techniques | | | | chain reaction, which is used to amplify and |
| of the 90's. In fact, it was THE technique that | | | | simultaneously quantify a targeted DNA molecule |
| completely revolutionized molecular biology. Its | | | | in a given sample. The qPCR applications include |
| been more than a decade of PCR domination in | | | | gene quantification, pathogen detection and |
| molecular biology and clinical diagnostic labs, | | | | validation of drug targets. Quantitation is not a |
| worldwide. The technique has evolved with these | | | | new idea in molecular biology. HIV viral loads in |
| passing years, and now, it is quantitative PCR | | | | AIDS patients have been analyzed since decades |
| (qPCR) which is getting the same attention and is | | | | now. These days, the focus is on quantitation |
| becoming one of the most widely used molecular | | | | because of the advancements in genomic |
| biology techniques of this new millennium. | | | | research. |
| Quantitative PCR, as the name suggests, is a | | | | |